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Thermo Scientific (Fermentas)限制性内切酶一览表

识别位点
孵育温度
推荐缓冲液
在各种Fermentas缓冲液中的活性
B
G
O
R
T
2xT
F
AanI
TTATAA
37  °C
T
50-100%
50-100%
0-20%
0-20%
100%
20-50%
 
AarI
CACCTGC
37  °C
Buffer  AarI + oligo
NR
NR
0-20%
0-20%
NR
50-100%
 
AasI
GACNNNNNNGTC
37  °C
B
100%
20-50%
0-20%
0-20%
50-100%
0-20%
 
AatII
GACGTC
37  °C
T
50-100%
20-50%
0-20%
0-20%
100%
20-50%
 
FastDigest® AatII
GACGTC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AccI
GTMKAC
37  °C
F
 
 
 
 
 
 
100%
Acc65I
GGTACC
37  °C
O
0-20%
20-50%
100%
20-50%
20-50%
50-100%
 
FastDigest® Acc65I
GGTACC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AciI
CCGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AclI
AACGTT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AcuI
CTGAAG
37  °C
F  + SAM
 
 
 
 
 
 
100%
AdeI
CACNNNGTG
37  °C
G
0-20%
100%
20-50%
100%
100%
20-50%
 
FastDigest® AfeI
AGCGCT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AflII
CTTAAG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AgeI
ACCGGT
37  °C
F
 
 
 
 
 
 
100%
AjiI
CACGTC
37  °C
Buffer  AjiI
NR
NR
20-50%
NR
NR
20-50%
 
AjuI
GAANNNNNNNTTGG
37  °C
R  + SAM
0-20%
50-100%
20-50%
100%
50-100%
50-100%
 
FastDigest® AjuI
GAANNNNNNNTTGG
37  °C
F  + SAM
 
 
 
 
 
 
100%
FastDigest® AleI
CACNNNNGTG
37  °C
F
 
 
 
 
 
 
100%
AlfI
GCANNNNNNTGC
37  °C
R  + SAM
0-20%
0-20%
0-20%
100%
0-20%
20-50%
 
AloI
GAACNNNNNNTCC
37  °C
R
0-20%
0-20%
0-20%
100%
20-50%
100%
 
AluI
AGCT
37  °C
T
50-100%
0-20%
0-20%
0-20%
100%
20-50%
 
FastDigest® AluI
AGCT
37  °C
F
 
 
 
 
 
 
100%
Alw21I
GWGCWC
37  °C
O
0-20%
20-50%
100%
50-100%
20-50%
50-100%
 
FastDigest® Alw21I
GWGCWC
37  °C
F
 
 
 
 
 
 
100%
Alw26I
GTCTC
37  °C
T
50-100%
100%
0-20%
0-20%
100%
100%
 
FastDigest® Alw26I
GTCTC
37  °C
F
 
 
 
 
 
 
100%
Alw44I
GTGCAC
37  °C
T
50-100%
100%
0-20%
50-100%
100%
50-100%
 
FastDigest® AlwNI
CAGNNNCTG
37  °C
F
 
 
 
 
 
 
100%
ApaI
GGGCCC
37  °C
B
100%
20-50%
0-20%
0-20%
20-50%
0-20%
 
FastDigest® ApaI
GGGCCC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® ApaLI
GTGCAC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AscI
GGCGCGCC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AseI
ATTAAT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AsiSI
GCGATCGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AvaI
CYCGRG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AvaII
GGWCC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® AvrII
CCTAGG
37  °C
F
 
 
 
 
 
 
100%
BamHI
GGATCC
37  °C
Buffer  BamHI
20-50%
100%
20-50%
50-100%
100%
50-100%
 
FastDigest® BamHI
GGATCC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BanI
GGYRCC
37  °C
F
 
 
 
 
 
 
100%
BauI
CACGAG
37  °C
T
0-20%
50-100%
0-20%
50-100%
100%
50-100%
 
FastDigest® BbsI
GAAGAC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BbvI
GCAGC
37  °C
2xT
 
 
 
 
 
100%
 
BclI
TGATCA
55  °C
G
20-50%
100%
20-50%
20-50%
100%
100%
 
FastDigest® BclI
TGATCA
37  °C
F
 
 
 
 
 
 
100%
BcnI
CCSGG
37  °C
T
20-50%
50-100%
50-100%
50-100%
100%
50-100%
 
BcuI
ACTAGT
37  °C
T
50-100%
50-100%
0-20%
20-50%
100%
0-20%
 
BdaI
TGANNNNNNTCA
30  °C
G  + SAM
NR
100%
0-20%
20-50%
50-100%
50-100%
 
FastDigest® BfaI
CTAG
37  °C
F
 
 
 
 
 
 
100%
BfiI
ACTGGG
37  °C
T
20-50%
20-50%
0-20%
0-20%
100%
0-20%
 
BfmI
CTRYAG
37  °C
T
0-20%
50-100%
0-20%
0-20%
100%
20-50%
 
BfuI
GTATCC
37  °C
Buffer  BfuI
NR
NR
0-20%
0-20%
NR
50-100%
 
BglI
GCCNNNNNGGC
37  °C
O
0-20%
50-100%
100%
100%
0-20%
100%
 
FastDigest® BglI
GCCNNNNNGGC
37  °C
F
 
 
 
 
 
 
100%
BglII
AGATCT
37  °C
O
0-20%
20-50%
100%
50-100%
0-20%
100%
 
FastDigest® BglII
AGATCT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BlpI
GCTNAGC
37  °C
F
 
 
 
 
 
 
100%
Bme1390I
CCNGG
37  °C
O
20-50%
50-100%
100%
50-100%
50-100%
50-100%
 
FastDigest® Bme1580I
GKGCMC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BmtI
GCTAGC
37  °C
F
 
 
 
 
 
 
100%
BoxI
GACNNNNGTC
37  °C
T
0-20%
0-20%
0-20%
20-50%
100%
20-50%
 
BpiI
GAAGAC
37  °C
G
20-50%
100%
50-100%
50-100%
50-100%
50-100%
 
BplI
GAGNNNNNCTC
37  °C
T  + SAM
0-20%
20-50%
0-20%
0-20%
100%
20-50%
 
FastDigest® BplI
GAGNNNNNCTC
37  °C
F  + SAM
 
 
 
 
 
 
100%
FastDigest® BpmI
CTGGAG
37  °C
F
 
 
 
 
 
 
100%
Bpu10I
CCTNAGC
37  °C
Buffer  Bpu10I
0-20%
20-50%
50-100%
100%
50-100%
100%
 
FastDigest® Bpu10I
CCTNAGC
37  °C
F
 
 
 
 
 
 
100%
Bpu1102I
GCTNAGC
37  °C
T
50-100%
50-100%
20-50%
20-50%
100%
20-50%
 
FastDigest® BsaAI
YACGTR
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BsaBI
GATNNNNATC
65  °C
F
 
 
 
 
 
 
100%
FastDigest® BsaHI
GRCGYC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BsaJI
CCNNGG
37  °C
F
 
 
 
 
 
 
100%
BseDI
CCNNGG
55  °C
T
50-100%
20-50%
0-20%
0-20%
100%
50-100%
 
BseGI
GGATG
55  °C
T
20-50%
50-100%
20-50%
20-50%
100%
20-50%
 
FastDigest® BseGI
GGATG
37  °C
F
 
 
 
 
 
 
100%
BseJI
GATNNNNATC
65  °C
O
NR
100%
100%
NR
NR
100%
 
BseLI
CCNNNNNNNGG
55  °C
T
20-50%
100%
50-100%
20-50%
100%
50-100%
 
BseMI
GCAATG
55  °C
R
0-20%
20-50%
0-20%
100%
50-100%
50-100%
 
BseMII
CTCAG
55  °C
T  + SAM
50-100%
50-100%
50-100%
50-100%
100%
50-100%
 
BseNI
ACTGG
65  °C
B
100%
20-50%
0-20%
0-20%
50-100%
20-50%
 
FastDigest® BseNI
ACTGG
65  °C
F
 
 
 
 
 
 
100%
BseSI
GKGCMC
55  °C
G
20-50%
100%
0-20%
20-50%
50-100%
0-20%
 
BseXI
GCAGC
65  °C
Buffer  BseXI
NR
NR
NR
NR
NR
NR
 
FastDigest® BseXI
GCAGC
65  °C
F
 
 
 
 
 
 
100%
Bsh1236I
CGCG
37  °C
R
0-20%
0-20%
50-100%
100%
20-50%
50-100%
 
FastDigest® Bsh1236I
CGCG
37  °C
F
 
 
 
 
 
 
100%
Bsh1285I
CGRYCG
37  °C
G
20-50%
100%
20-50%
0-20%
0-20%
20-50%
 
BshNI
GGYRCC
37  °C
O
0-20%
20-50%
100%
50-100%
0-20%
100%
 
BshTI
ACCGGT
37  °C
O
0-20%
20-50%
100%
50-100%
20-50%
20-50%
 
FastDigest® BsiEI
CGRYCG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BsiWI
CGTACG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BslI
CCNNNNNNNGG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BsmBI
CGTCTC
37  °C
F  + DTT
 
 
 
 
 
 
100%
FastDigest® BsmFI
GGGAC
37  °C
F  + SAM
 
 
 
 
 
 
100%
Bsp68I
TCGCGA
37  °C
O
0-20%
20-50%
100%
50-100%
20-50%
50-100%
 
Bsp119I
TTCGAA
37  °C
T
20-50%
0-20%
0-20%
0-20%
100%
100%
 
FastDigest® Bsp119I
TTCGAA
37  °C
F
 
 
 
 
 
 
100%
Bsp120I
GGGCCC
37  °C
B
100%
20-50%
0-20%
20-50%
50-100%
0-20%
 
FastDigest® Bsp120I
GGGCCC
37  °C
F
 
 
 
 
 
 
100%
Bsp143I
GATC
37  °C
Buffer  Bsp143I
20-50%
20-50%
0-20%
0-20%
50-100%
20-50%
 
FastDigest® Bsp1286I
GDGCHC
37  °C
F
 
 
 
 
 
 
100%
Bsp1407I
TGTACA
37  °C
T
0-20%
20-50%
0-20%
20-50%
100%
50-100%
 
FastDigest® Bsp1407I
TGTACA
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BspCNI
CTCAG
37  °C
F  + SAM
 
 
 
 
 
 
100%
FastDigest® BspHI
TCATGA
37  °C
F
 
 
 
 
 
 
100%
BspLI
GGNNCC
37  °C
T
50-100%
50-100%
0-20%
20-50%
100%
20-50%
 
FastDigest® BspMI
ACCTGC
37  °C
F
 
 
 
 
 
 
100%
BspOI
GCTAGC
37  °C
O
0-20%
0-20%
100%
100%
0-20%
20-50%
 
BspPI
GGATC
55  °C
T
20-50%
20-50%
0-20%
0-20%
100%
0-20%
 
BspTI
CTTAAG
37  °C
O
0-20%
0-20%
100%
20-50%
0-20%
50-100%
 
FastDigest® BsrBI
CCGCTC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BsrDI
GCAATG
55  °C
F
 
 
 
 
 
 
100%
FastDigest® BsrFI
RCCGGY
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BssHII
GCGCGC
37  °C
F
 
 
 
 
 
 
100%
Bst1107I
GTATAC
37  °C
O
20-50%
50-100%
100%
100%
20-50%
100%
 
BstXI
CCANNNNNNTGG
55  °C
O
20-50%
100%
100%
50-100%
50-100%
100%
 
FastDigest® BstXI
CCANNNNNNTGG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® BstZ17I
GTATAC
37  °C
F
 
 
 
 
 
 
100%
Bsu15I
ATCGAT
37  °C
T
20-50%
20-50%
20-50%
20-50%
100%
20-50%
 
FastDigest® Bsu36I
CCTNAGG
37  °C
F
 
 
 
 
 
 
100%
BsuRI
GGCC
37  °C
R
20-50%
20-50%
50-100%
100%
50-100%
100%
 
BveI
ACCTGC
37  °C
O  + oligo
0-20%
20-50%
100%
20-50%
50-100%
100%
 
CaiI
CAGNNNCTG
37  °C
T
20-50%
20-50%
20-50%
50-100%
100%
50-100%
 
CfrI
YGGCCR
37  °C
T
50-100%
50-100%
0-20%
0-20%
100%
0-20%
 
Cfr9I
CCCGGG
37  °C
Buffer  Cfr9I
0-20%
0-20%
0-20%
0-20%
20-50%
0-20%
 
Cfr10I
RCCGGY
37  °C
Buffer  Cfr10I
0-20%
20-50%
20-50%
50-100%
20-50%
50-100%
 
Cfr13I
GGNCC
37  °C
T
50-100%
50-100%
20-50%
20-50%
100%
20-50%
 
Cfr42I
CCGCGG
37  °C
B
100%
50-100%
0-20%
0-20%
50-100%
0-20%
 
FastDigest® ClaI
ATCGAT
37  °C
F
 
 
 
 
 
 
100%
CpoI
CGGWCCG
37  °C
T
20-50%
50-100%
50-100%
20-50%
100%
50-100%
 
CseI
GACGC
37  °C
R
NR
50-100%
50-100%
100%
100%
50-100%
 
Csp6I
GTAC
37  °C
B
100%
50-100%
0-20%
0-20%
50-100%
0-20%
 
FastDigest® Csp6I
GTAC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® DdeI
CTNAG
37  °C
F
 
 
 
 
 
 
100%
DpnI
GATC
37  °C
T
100%
100%
50-100%
50-100%
100%
50-100%
 
FastDigest® DpnI
GATC
37  °C
F
 
 
 
 
 
 
100%
DraI
TTTAAA
37  °C
T
50-100%
50-100%
20-50%
20-50%
100%
50-100%
 
FastDigest® DraI
TTTAAA
37  °C
F
 
 
 
 
 
 
100%
FastDigest® DraIII
CACNNNGTG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® DrdI
GACNNNNNNGTC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® EagI
CGGCCG
37  °C
F
 
 
 
 
 
 
100%
Eam1104I
CTCTTC
37  °C
T
50-100%
50-100%
0-20%
0-20%
100%
0-20%
 
Eam1105I
GACNNNNNGTC
37  °C
Buffer  Eam1105I
20-50%
50-100%
0-20%
0-20%
50-100%
20-50%
 
FastDigest® Eam1105I
GACNNNNNGTC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® EarI
CTCTTC
37  °C
F
 
 
 
 
 
 
100%
Ecl136II
GAGCTC
37  °C
Buffer  Ecl136II
50-100%
20-50%
0-20%
0-20%
50-100%
0-20%
 
FastDigest® Ecl136II
GAGCTC
37  °C
F
 
 
 
 
 
 
100%
Eco24I
GRGCYC
37  °C
T
50-100%
50-100%
0-20%
20-50%
100%
0-20%
 
Eco31I
GGTCTC
37  °C
G
50-100%
100%
0-20%
0-20%
50-100%
20-50%
 
FastDigest® Eco31I
GGTCTC
37  °C
F
 
 
 
 
 
 
100%
Eco32I
GATATC
37  °C
R
0-20%
50-100%
50-100%
100%
20-50%
100%
 
Eco47I
GGWCC
37  °C
R
0-20%
50-100%
50-100%
100%
50-100%
50-100%
 
Eco47III
AGCGCT
37  °C
O
0-20%
20-50%
100%
100%
50-100%
100%
 
Eco52I
CGGCCG
37  °C
Buffer  Eco52I
0-20%
0-20%
0-20%
20-50%
0-20%
20-50%
 
Eco57I
CTGAAG
37  °C
G  + SAM
100%
100%
20-50%
20-50%
50-100%
50-100%
 
Eco57MI
CTGRAG
37  °C
B  + SAM
100%
50-100%
0-20%
20-50%
50-100%
0-20%
 
Eco72I
CACGTG
37  °C
T
NR
NR
0-20%
0-20%
100%
20-50%
 
Eco81I
CCTNAGG
37  °C
T
50-100%
100%
0-20%
0-20%
100%
0-20%
 
Eco88I
CYCGRG
37  °C
T
100%
50-100%
0-20%
0-20%
100%
20-50%
 
Eco91I
GGTNACC
37  °C
O
20-50%
20-50%
100%
50-100%
5-100%
100%
 
FastDigest® Eco91I
GGTNACC
37  °C
F
 
 
 
 
 
 
100%
Eco105I
TACGTA
37  °C
T
100%
50-100%
0-20%
0-20%
100%
0-20%
 
Eco130I
CCWWGG
37  °C
O
0-20%
20-50%
100%
50-100%
50-100%
100%
 
Eco147I
AGGCCT
37  °C
B
100%
50-100%
20-50%
20-50%
50-100%
0-20%
 
FastDigest® EcoNI
CCTNNNNNAGG
37  °C
F
 
 
 
 
 
 
100%
EcoO109I
RGGNCCY
37  °C
T
50-100%
20-50%
20-50%
20-50%
100%
100%
 
FastDigest® EcoO109I
RGGNCCY
37  °C
F
 
 
 
 
 
 
100%
EcoRI
GAATTC
37  °C
Buffer  EcoRI
0-20%
NR
100%
100%
NR
100%
 
FastDigest® EcoRI
GAATTC
37  °C
F
 
 
 
 
 
 
100%
EcoRII
CCWGG
37  °C
O
20-50%
50-100%
100%
50-100%
20-50%
50-100%
 
FastDigest® EcoRV
GATATC
37  °C
F
 
 
 
 
 
 
100%
EheI
GGCGCC
37  °C
T
20-50%
50-100%
0-20%
0-20%
100%
20-50%
 
FastDigest® EheI
GGCGCC
37  °C
F
 
 
 
 
 
 
100%
Esp3I
CGTCTC
37  °C
T  + DTT
100%
20-50%
0-20%
0-20%
100%
0-20%
 
FaqI
GGGAC
37  °C
T  + SAM
20-50%
20-50%
0-20%
0-20%
100%
20-50%
 
FastDigest® Fnu4HI
GCNGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® FokI
GGATG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® FspI
TGCGCA
37  °C
F
 
 
 
 
 
 
100%
FspAI
RTGCGCAY
37  °C
O
0-20%
0-20%
100%
50-100%
0-20%
50-100%
 
FastDigest® FspAI
RTGCGCAY
37  °C
F
 
 
 
 
 
 
100%
FspBI
CTAG
37  °C
T
50-100%
20-50%
0-20%
0-20%
100%
0-20%
 
GsuI
CTGGAG
30  °C
B
100%
50-100%
20-50%
20-50%
100%
50-100%
 
FastDigest® HaeII
RGCGCY
37  °C
F
 
 
 
 
 
 
100%
FastDigest® HaeIII
GGCC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® HgaI
GACGC
37  °C
F
 
 
 
 
 
 
100%
HhaI
GCGC
37  °C
T
50-100%
50-100%
20-50%
20-50%
100%
20-50%
 
FastDigest® HhaI
GCGC
37  °C
F
 
 
 
 
 
 
100%
Hin1I
GRCGYC
37  °C
G
20-50%
100%
20-50%
20-50%
20-50%
20-50%
 
Hin1II
CATG
37  °C
G
50-100%
100%
20-50%
50-100%
50-100%
50-100%
 
Hin4I
GAYNNNNNVTC
37  °C
T  + SAM
20-50%
20-50%
0-20%
0-20%
100%
0-20%
 
Hin6I
GCGC
37  °C
T
50-100%
50-100%
20-50%
20-50%
100%
50-100%
 
HincII
GTYRAC
37  °C
T
50-100%
50-100%
20-50%
50-100%
100%
50-100%
 
FastDigest® HincII
GTYRAC
37  °C
F
 
 
 
 
 
 
100%
HindIII
AAGCTT
37  °C
R
0-20%
20-50%
0-20%
100%
50-100%
50-100%
 
FastDigest® HindIII
AAGCTT
37  °C
F
 
 
 
 
 
 
100%
HinfI
GANTC
37  °C
R
0-20%
20-50%
50-100%
100%
50-100%
50-100%
 
FastDigest® HinfI
GANTC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® HinP1I
GCGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® HpaI
GTTAAC
37  °C
F
 
 
 
 
 
 
100%
HpaII
CCGG
37  °C
T
50-100%
50-100%
0-20%
20-50%
100%
20-50%
 
FastDigest® HpaII
CCGG
37  °C
F
 
 
 
 
 
 
100%
HphI
GGTGA
37  °C
B
100%
0-20%
0-20%
0-20%
20-50%
0-20%
 
Hpy8I
GTNNAC
37  °C
T
50-100%
50-100%
0-20%
20-50%
100%
50-100%
 
FastDigest® Hpy8I
GTNNAC
37  °C
F
 
 
 
 
 
 
100%
HpyF3I
CTNAG
37  °C
T
20-50%
20-50%
20-50%
20-50%
100%
50-100%
 
HpyF10VI
GCNNNNNNNGC
37  °C
T
0-20%
0-20%
0-20%
0-20%
100%
50-100%
 
FastDigest® HpyF10VI
GCNNNNNNNGC
37  °C
F
 
 
 
 
 
 
100%
I-SceI
TAGGGATAACAGGGTAAT
37  °C
T
50-100%
50-100%
50-100%
50-100%
100%
50-100%
 
KpnI
GGTACC
37  °C
Buffer  KpnI
20-50%
0-20%
0-20%
0-20%
20-50%
0-20%
 
FastDigest® KpnI
GGTACC
37  °C
F
 
 
 
 
 
 
100%
Kpn2I
TCCGGA
55  °C
T
50-100%
50-100%
0-20%
20-50%
100%
50-100%
 
FastDigest® Kpn2I
TCCGGA
37  °C
F
 
 
 
 
 
 
100%
KspAI
GTTAAC
37  °C
B
100%
50-100%
20-50%
20-50%
100%
50-100%
 
LguI
GCTCTTC
37  °C
T
20-50%
50-100%
20-50%
20-50%
100%
20-50%
 
Lsp1109I
GCAGC
37  °C
Buffer  Lsp1109I
0-20%
20-50%
50-100%
100%
20-50%
20-50%
 
LweI
GCATC
37  °C
T
0-20%
0-20%
0-20%
20-50%
100%
20-50%
 
MauBI
CGCGCGCG
37  °C
T
0-20%
0-20%
0-20%
0-20%
100%
0-20%
 
FastDigest® MauBI
CGCGCGCG
37  °C
F
 
 
 
 
 
 
100%
MbiI
GAGCGG
37  °C
T
20-50%
100%
20-50%
20-50%
100%
20-50%
 
MboI
GATC
37  °C
R
50-100%
50-100%
50-100%
100%
50-100%
100%
 
FastDigest® MboI
GATC
37  °C
F
 
 
 
 
 
 
100%
MboII
GAAGA
37  °C
B
100%
50-100%
20-50%
0-20%
50-100%
20-50%
 
FastDigest® MboII
GAAGA
37  °C
F
 
 
 
 
 
 
100%
FastDigest® MfeI
CAATTG
37  °C
F
 
 
 
 
 
 
100%
MlsI
TGGCCA
37  °C
R
0-20%
20-50%
0-20%
100%
20-50%
50-100%
 
MluI
ACGCGT
37  °C
R
0-20%
20-50%
50-100%
100%
20-50%
50-100%
 
FastDigest® MluI
ACGCGT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® MlyI
GAGTC
37  °C
F
 
 
 
 
 
 
100%
MnlI
CCTC
37  °C
G
50-100%
100%
20-50%
20-50%
20-50%
20-50%
 
FastDigest® MnlI
CCTC
37  °C
F
 
 
 
 
 
 
100%
Mph1103I
ATGCAT
37  °C
R
0-20%
50-100%
20-50%
100%
50-100%
50-100%
 
MreI
CGCCGGCG
37  °C
G
20-50%
100%
0-20%
0-20%
50-100%
0-20%
 
FastDigest® MreI
CGCCGGCG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® MscI
TGGCCA
37  °C
F
 
 
 
 
 
 
100%
FastDigest® MseI
TTAA
37  °C
F
 
 
 
 
 
 
100%
FastDigest® MslI
CAYNNNNRTG
37  °C
F
 
 
 
 
 
 
100%
MspI
CCGG
37  °C
T
50-100%
50-100%
0-20%
0-20%
100%
50-100%
 
FastDigest® MspI
CCGG
37  °C
F
 
 
 
 
 
 
100%
MssI
GTTTAAAC
37  °C
B
100%
0-20%
0-20%
0-20%
20-50%
0-20%
 
FastDigest® MssI
GTTTAAAC
37  °C
F
 
 
 
 
 
 
100%
MunI
CAATTG
37  °C
G
100%
100%
0-20%
0-20%
100%
0-20%
 
MvaI
CCWGG
37  °C
R
20-50%
20-50%
50-100%
100%
20-50%
100%
 
FastDigest® MvaI
CCWGG
37  °C
F
 
 
 
 
 
 
100%
Mva1269I
GAATGC
37  °C
R
0-20%
20-50%
50-100%
100%
0-20%
50-100%
 
FastDigest® Mva1269I
GAATGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® NaeI
GCCGGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® NciI
CCSGG
37  °C
F
 
 
 
 
 
 
100%
NcoI
CCATGG
37  °C
T
20-50%
20-50%
20-50%
50-100%
100%
100%
 
FastDigest® NcoI
CCATGG
37  °C
F
 
 
 
 
 
 
100%
NdeI
CATATG
37  °C
O
0-20%
0-20%
100%
50-100%
0-20%
50-100%
 
FastDigest® NdeI
CATATG
37  °C
F
 
 
 
 
 
 
100%
NheI
GCTAGC
37  °C
T
100%
20-50%
0-20%
0-20%
100%
0-20%
 
FastDigest® NheI
GCTAGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® NlaIII
CATG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® NlaIV
GGNNCC
37  °C
F
 
 
 
 
 
 
100%
NmuCI
GTSAC
37  °C
R
0-20%
20-50%
50-100%
100%
20-50%
50-100%
 
FastDigest® NmuCI
GTSAC
37  °C
F
 
 
 
 
 
 
100%
NotI
GCGGCCGC
37  °C
O
0-20%
0-20%
100%
20-50%
0-20%
20-50%
 
FastDigest® NotI
GCGGCCGC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® NruI
TCGCGA
37  °C
F
 
 
 
 
 
 
100%
NsbI
TGCGCA
37  °C
T
20-50%
50-100%
0-20%
20-50%
100%
20-50%
 
FastDigest® NsiI
ATGCAT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® NspI
RCATGY
37  °C
F
 
 
 
 
 
 
100%
OliI
CACNNNNGTG
37  °C
R
0-20%
0-20%
0-20%
100%
0-20%
50-100%
 
PacI
TTAATTAA
37  °C
Buffer  PacI
20-50%
20-50%
0-20%
0-20%
0-20%
0-20%
 
FastDigest® PacI
TTAATTAA
37  °C
F
 
 
 
 
 
 
100%
PaeI
GCATGC
37  °C
B
100%
50-100%
0-20%
0-20%
50-100%
0-20%
 
PagI
TCATGA
37  °C
O
0-20%
50-100%
100%
NR
NR
NR
 
PasI
CCCWGGG
55  °C
Buffer  PasI
NR
NR
NR
NR
NR
NR
 
PauI
GCGCGC
37  °C
R
0-20%
0-20%
100%
100%
0-20%
100%
 
PdiI
GCCGGC
37  °C
T
50-100%
20-50%
0-20%
0-20%
100%
50-100%
 
PdmI
GAANNNNTTC
37  °C
T
20-50%
50-100%
0-20%
0-20%
100%
0-20%
 
FastDigest® PdmI
GAANNNNTTC
37  °C
F
 
 
 
 
 
 
100%
PfeI
GAWTC
37  °C
O
0-20%
20-50%
100%
50-100%
20-50%
50-100%
 
Pfl23II
CGTACG
37  °C
T
20-50%
50-100%
20-50%
20-50%
100%
0-20%
 
FastDigest® PflMI
CCANNNNNTGG
37  °C
F
 
 
 
 
 
 
100%
PfoI
TCCNGGA
37  °C
T
0-20%
20-50%
50-100%
0-20%
100%
50-100%
 
FastDigest® PfoI
TCCNGGA
37  °C
F
 
 
 
 
 
 
100%
FastDigest® PmlI
CACGTG
37  °C
F
 
 
 
 
 
 
100%
PpiI
GAACNNNNNCTC
30  °C
R
0-20%
0-20%
0-20%
100%
50-100%
50-100%
 
Ppu21I
YACGTR
30  °C
Buffer  Ppu21I
50-100%
100%
20-50%
NR
NR
NR
 
FastDigest® PpuMI
RGGWCCY
37  °C
F
 
 
 
 
 
 
100%
PscI
ACATGT
37  °C
T
20-50%
20-50%
0-20%
0-20%
100%
0-20%
 
FastDigest® PshAI
GACNNNNGTC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® PsiI
TTATAA
37  °C
F
 
 
 
 
 
 
100%
Psp5II
RGGWCCY
37  °C
G
0-20%
100%
20-50%
20-50%
50-100%
100%
 
Psp1406I
AACGTT
37  °C
T
100%
50-100%
0-20%
20-50%
100%
0-20%
 
FastDigest® PspFI
CCCAGC
37  °C
F
 
 
 
 
 
 
100%
PstI
CTGCAG
37  °C
O
50-100%
50-100%
100%
100%
50-100%
50-100%
 
FastDigest® PstI
CTGCAG
37  °C
F
 
 
 
 
 
 
100%
PsuI
RGATCY
37  °C
B
100%
20-50%
0-20%
0-20%
50-100%
0-20%
 
FastDigest® PsuI
RGATCY
37  °C
F
 
 
 
 
 
 
100%
PsyI
GACNNNGTC
37  °C
B
100%
50-100%
0-20%
0-20%
50-100%
0-20%
 
FastDigest® PsyI
GACNNNGTC
37  °C
F
 
 
 
 
 
 
100%
PvuI
CGATCG
37  °C
R
0-20%
20-50%
50-100%
100%
50-100%
100%
 
FastDigest® PvuI
CGATCG
37  °C
F
 
 
 
 
 
 
100%
PvuII
CAGCTG
37  °C
G
50-100%
100%
20-50%
50-100%
20-50%
20-50%
 
FastDigest® PvuII
CAGCTG
37  °C
F
 
 
 
 
 
 
100%
RsaI
GTAC
37  °C
T
50-100%
20-50%
0-20%
0-20%
100%
0-20%
 
FastDigest® RsaI
GTAC
37  °C
F
 
 
 
 
 
 
100%
RseI
CAYNNNNRTG
37  °C
R
0-20%
50-100%
50-100%
100%
20-50%
100%
 
FastDigest® RsrII
CGGWCCG
37  °C
F
 
 
 
 
 
 
100%
SacI
GAGCTC
37  °C
Buffer  SacI
50-100%
20-50%
0-20%
0-20%
50-100%
20-50%
 
FastDigest® SacI
GAGCTC
37  °C
F
 
 
 
 
 
 
100%
SalI
GTCGAC
37  °C
O
0-20%
0-20%
100%
20-50%
0-20%
50-100%
 
FastDigest® SalI
GTCGAC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® SanDI
GGGWCCC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® SapI
GCTCTTC
37  °C
F
 
 
 
 
 
 
100%
SatI
GCNGC
37  °C
G
20-50%
100%
20-50%
20-50%
50-100%
20-50%
 
FastDigest® Sau3AI
GATC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® Sau96I
GGNCC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® SbfI
CCTGCAGG
37  °C
F
 
 
 
 
 
 
100%
ScaI
AGTACT
37  °C
Buffer  ScaI
0-20%
0-20%
0-20%
0-20%
0-20%
0-20%
 
FastDigest® ScaI
AGTACT
37  °C
F
 
 
 
 
 
 
100%
SchI
GAGTC
37  °C
T
20-50%
50-100%
0-20%
0-20%
100%
0-20%
 
FastDigest® ScrFI
CCNGG
37  °C
F
 
 
 
 
 
 
100%
SdaI
CCTGCAGG
37  °C
Buffer  SdaI
NR
NR
0-20%
0-20%
NR
20-50%
 
SduI
GDGCHC
37  °C
Buffer  SduI
NR
50-100%
50-100%
0-20%
NR
NR
 
FastDigest® SexAI
ACCWGGT
37  °C
F
 
 
 
 
 
 
100%
SfaAI
GCGATCGC
37  °C
T
50-100%
0-20%
0-20%
0-20%
100%
0-20%
 
FastDigest® SfaNI
GCATC
37  °C
F
 
 
 
 
 
 
100%
FastDigest® SfcI
CTRYAG
37  °C
F
 
 
 
 
 
 
100%
SfiI
GGCCNNNNNGGCC
50  °C
G
50-100%
100%
20-50%
0-20%
100%
0-20%
 
FastDigest® SfiI
GGCCNNNNNGGCC
50  °C
F
 
 
 
 
 
 
100%
SgrDI
CGTCGACG
37  °C
R
0-20%
0-20%
0-20%
100%
NR
100%
 
SgsI
GGCGCGCC
37  °C
T
0-20%
0-20%
0-20%
50-100%
100%
50-100%
 
SmaI
CCCGGG
30  °C
T
50-100%
0-20%
0-20%
0-20%
100%
0-20%
 
FastDigest® SmaI
CCCGGG
37  °C
F
 
 
 
 
 
 
100%
SmiI
ATTTAAAT
30  °C
O
0-20%
0-20%
100%
20-50%
0-20%
20-50%
 
SmoI
CTYRAG
55  °C
T
50-100%
20-50%
0-20%
20-50%
100%
20-50%
 
SmuI
CCCGC
37  °C
T
50-100%
50-100%
0-20%
20-50%
100%
20-50%
 
FastDigest® SnaBI
TACGTA
37  °C
F
 
 
 
 
 
 
100%
FastDigest® SpeI
ACTAGT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® SphI
GCATGC
37  °C
F
 
 
 
 
 
 
100%
SsiI
CCGC
37  °C
O
NR
20-50%
100%
50-100%
NR
100%
 
SspI
AATATT
37  °C
G
20-50%
100%
0-20%
50-100%
100%
20-50%
 
FastDigest® SspI
AATATT
37  °C
F
 
 
 
 
 
 
100%
SspDI
GGCGCC
37  °C
T
20-50%
0-20%
NR
0-20%
100%
20-50%
 
FastDigest® StuI
AGGCCT
37  °C
F
 
 
 
 
 
 
100%
FastDigest® StyI
CCWWGG
37  °C
F
 
 
 
 
 
 
100%
FastDigest® SwaI
ATTTAAAT
37  °C
F
 
 
 
 
 
 
100%
TaaI
ACNGT
65  °C
T
0-20%
0-20%
0-20%
50-100%
100%
100%
 
FastDigest® TaaI
ACNGT
65  °C
F
 
 
 
 
 
 
100%
TaiI
ACGT
65  °C
R
50-100%
50-100%
20-50%
100%
100%
50-100%
 
FastDigest® TaiI
ACGT
65  °C
F
 
 
 
 
 
 
100%
TaqI
TCGA
65  °C
Buffer  TaqI
0-20%
20-50%
20-50%
20-50%
20-50%
20-50%
 
FastDigest® TaqI
TCGA
65  °C
F
 
 
 
 
 
 
100%
TasI
AATT
65  °C
B
100%
50-100%
20-50%
0-20%
20-50%
0-20%
 
TatI
WGTACW
65  °C
T
NR
50-100%
20-50%
20-50%
100%
0-20%
 
FastDigest® TatI
WGTACW
65  °C
F
 
 
 
 
 
 
100%
TauI
GCSGC
55  °C
B
100%
50-100%
0-20%
0-20%
20-50%
0-20%
 
FastDigest® TauI
GCSGC
55  °C
F
 
 
 
 
 
 
100%
FastDigest® TfiI
GAWTC
37  °C
F
 
 
 
 
 
 
100%
Tru1I
TTAA
65  °C
R
50-100%
50-100%
20-50%
100%
50-100%
100%
 
FastDigest® Tru1I
TTAA
65  °C
F
 
 
 
 
 
 
100%
TscAI
CASTG
65  °C
T
50-100%
50-100%
20-50%
20-50%
100%
20-50%
 
TsoI
TARCCA
55  °C
G  + SAM
NR
100%
50-100%
0-20%
50-100%
20-50%
 
FastDigest® Tsp509I
AATT
65  °C
F
 
 
 
 
 
 
100%
FastDigest® TspRI
CASTG
37  °C
F
 
 
 
 
 
 
100%
TstI
CACNNNNNNTCC
37  °C
R
0-20%
0-20%
0-20%
100%
0-20%
100%
 
Van91I
CCANNNNNTGG
37  °C
R
0-20%
50-100%
50-100%
100%
20-50%
50-100%
 
VspI
ATTAAT
37  °C
O
0-20%
50-100%
100%
20-50%
100%
100%
 
XagI
CCTNNNNNAGG
37  °C
R
0-20%
20-50%
50-100%
100%
20-50%
50-100%
 
XapI
RAATTY
37  °C
T
50-100%
100%
0-20%
0-20%
100%
20-50%
 
FastDigest® XapI
RAATTY
37  °C
F
 
 
 
 
 
 
100%
XbaI
TCTAGA
37  °C
T
50-100%
50-100%
20-50%
0-20%
100%
50-100%
 
FastDigest® XbaI
TCTAGA
37  °C
F
 
 
 
 
 
 
100%
XceI
RCATGY
37  °C
T
50-100%
0-20%
0-20%
0-20%
100%
0-20%
 
XhoI
CTCGAG
37  °C
R
0-20%
50-100%
50-100%
100%
20-50%
100%
 
FastDigest® XhoI
CTCGAG
37  °C
F
 
 
 
 
 
 
100%
XmaJI
CCTAGG
37  °C
T
20-50%
50-100%
50-100%
50-100%
100%
50-100%
 
XmiI
GTMKAC
37  °C
B
100%
0-20%
0-20%
0-20%
50-100%
20-50%
 
乐研生物 www.loybio.com)

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共 1 个关于【Thermo Scientific(Fermentas)限制性内切酶一览表】的回复 最后回复于 2014-3-21 21:37

苦丁茶 发表于 2014-3-21 21:37:41 | 显示全部楼层
如果可以出一个查询工具就好了,最好再把不同公司的整合到一起!
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